The Great Britain wheelchair racer could not have got his tactics more right as he showed no signs of tiredness from his 5,000m - T54 triumph two days earlier to successfully defend his 1500m - T54 title.
The 33-year-old was content to tuck in just off the lead for most of the race before hitting the front with around 300m to go and then holding off his rivals down the home straight to deafening cheers.
He crossed the line in 3:12.09, producing a 44.84 last lap to finish 0.23 ahead of Thailand's Prawat Wahoram. Republic of Korea's Kim Gyu Dae won bronze.
He said: 'I'm very proud. I'm shocked really because the field in the 1500m this year has been so strong and I've only won a couple of races, so coming into this race I was probably the fourth fastest on paper.
'But the training I've done, I knew I had lots of top speed. And on the warm-up track tonight, I was even quicker on the top speed. I wasn't so nervous tonight.'
Meanwhile, Algeria's Nassima Saifi claimed gold in the women's Discus - F57/58 with a best effort of 40.34m (1004) - a Paralympic record. Bulgaria's Stela Eneva took silver and Ireland's Orla Barry bronze.
Russia's Evgenii Shvetcov shattered the world record to win his second gold medal of the Games, adding the 400m - T36 title to his 100m crown.
The 24-year-old clocked 53.31, slicing 0.82s off the previous mark, to claim a comprehensive victory. Silver went to Briton Paul Blake, with Ukraine's Roman Pavlyk getting the bronze.
Ukraine's Ruslan Katyshev claimed the Long Jump - F11 gold with a leap of 6.46m. USA's Elexis Gillette picked up silver, with China's Li Duan getting bronze.
France's Assia El Hannouni won her seventh Paralympic gold medal with victory in the 400m - T12, while Brazil's Felipe Gomes won 200m - T11 gold.
Tunisia's Abderrahim Zhiou won the 1500m - T13 final as the top two finishers both set world records. Zhiou, a T12 athlete, clocked 3:48.31 ahead of T13 runner David Korir of Kenya, who came home in 3:48.84. Great Britain's David Devine was third.
There was further reason for Tunisian celebration with Walid Ktila storming to 200m - T34 gold in a world record 27.98.
Russia's Fedor Trikolich won the 100m - T12, and compatriot Elena Pautova the 1500m - T12.
Austria's Gunther Matzinger won the 400m - T46 and Spain's Jose Antonio Exposito Pineiro the Long Jump - F20 title.
Iran's Peyman Nasiri Bazanjani won the 1500m - T20, Kenya's Abraham Tarbei the 1500m - T46 and Morocco's Azeddine Nouiri the Shot Put - F34.
China's Wang Zhiming won the Discus - F40, and Russia the women's 4 x 100m Relay - T35-38 gold.
Alexey Ashapatov added Shot Put gold to his Discus title in the F57/58 class as he successfully defended his double from Beijing.
China's Mi Na won the Shot Put - F37 and Jamaican's Alphanso Cunningham the Javelin - F52/53.