The 34-year-old won two titles in the Velodrome and added gold in the 16km race against the clock - taking her total tally of Paralympic golds to 10 - on a course which included all the fabled sections of the motor-racing circuit.
Storey finished in 22:40 and will tomorrow ride in the women's Individual Road Race C4-5 seeking to level Tanni Grey-Thompson's haul of 11 Paralympic golds for Great Britain.
Poland's Anna Harkowska took silver and Kelly Crowley of the USA took bronze.
Storey's Paralympic career began as a 14-year-old in Barcelona and she won five titles as a swimmer before a series of ear infections saw her turn her attentions to the bike between Athens 2004 and Beijing 2008.
Following two golds in 2008, including in the Time Trial, Storey now has a hat-trick on home soil in her sixth Games.
She said: 'I'm absolutely delighted. It's just amazing to get around the circuit so quick. The first thing I'm going to do is see where I would have placed in the men's race.'