The South African was a hugely convincing winner of the 400m - T44 in a new Paralympic record 46.68, more than three seconds ahead of the field.
After a shock 200m loss and an expected 100m one, the 400m was the one title the 25-year-old wanted to keep hold of more than any other.
Pistorius came into the race with a personal best more than five seconds quicker than the next fastest in the race, none of whom had broken the 50-second barrier. Pistorius can get close to 45 at his best.
Alan Fonteles Oliveira, the 200m - T44 champion, was in the lane outside him and for the first half of the race looked like he might pose a challenge.
But Pistorius came into the home straight with a clear lead and, as the Brazilian tired badly, USA athlete Blake Leeper came into second and compatriot David Prince third. Oliveira was fourth.
Pistorius said: 'For one of the first times I thought I could actually hear something coming into the home straight.
'Usually I'm so focused and so in my zone, but I could hear the crowd and it was just the most amazing feeling that I could have possibly had.
'This has been the most phenomenonally successful Olympic and Paralympic Games and I think the world is finally seeing that Paralympic sport is truly elite.'
Tatyana McFadden saw her bid for a possible five Paralympic gold medals end with a third-placed finish in the 100m -T54. Chinese racers Liu Wenjun and Dong Hongjiao took gold and silver.
Fanie van der Merwe took the 100m - T37 title thanks to a spectacular dive for the line.
Van der Merwe and China's Liang Yongbin were both given 11.51, a world record, but the South African was awarded the gold in a photo finish.
Cuba's Yunidis Castillo ran a world record 55.72 to claim her third gold of the Games with victory in the 400m - T46.
Poland's Mateusz Michalski also set a new world record, clocking 21.56 to clinch the 200m - T12 crown, while his compatriot Maciej Lepiato quickly followed suit, taking the High Jump - F46 mark to new heights with a leap of 2.12m to take the gold.
Wheelchair racer Raymond Martin won gold number four by taking the 200m - T52 title.
Austria's Gunther Matzinger made it a 400m-800m double with victory in the 800m - T46 in a world record 1:51.82, while Russia's Elena Ivanova won the 100m - T36 and China's Xue Lei the 100m - T11.
China's 4 x 400m Relay - T53/54 quartet claimed a convincing gold, while Zhou Hongzhuan was crowned 400m - T53 champion and Mohammad Khalvandi took the Javelin - F57/58 gold.
Tunisian pair Walid Ktila and Raoua Tlili took the 100m - T34 crown and Shot Put - F40 title respectively.
Algeria's Abdellatif Baka timed his finish to perfection to snatch gold in the 800m - T13.
Australia's Evan O'Hanlon won his second gold of the Games as he added the 200m - T38 title to his 100m crown.
Mexico's Angeles Ortiz Hernandez won the Shot Put - F57/58 gold and compatriot Luis Alberto Zepeda Felix the Javelin - F54/55/56 title.
Tunisia's Neda Bahi won the 400m - T37, Brazil's Shirlene Coelho the Javelin - F37/38 and Azerbaijan's Oleg Panyutin the Triple Jump - F12.