The Australian had made her intentions clear this morning when she led the heats by 21 seconds.
Tonight she returned to the Aquatics Centre to claim her sixth individual and seventh title overall almost 20 seconds clear of Cortney Jordan in second, with Great Britain's Susie Rodgers in third.
The 20-year-old led from start to finish, touching in 4:59.02, 19.53 ahead of USA swimmer Jordan, to lower her own world record from December 2011 by 0.93.
Freney said: 'Seven. I'm in heaven. It's just unbelievable how good this meet's been for me. It's probably been one of my best meets to date. I couldn't be more proud of myself.'
Seven. I'm in heaven. It's just unbelievable how good this meet's been for me. It's probably been one of my best meets to date. I couldn't be more proud of myself.Jacqueline Freney
It brings to 10 her total number of Paralympic medals after her three bronzes in Beijing, with the 4 x 100m Medley Relay remaining.
Ordinarily, Jessica Long would be commanding the headlines as she claimed her fifth title with victory in the 100m Freestyle - S8 in world record time.
The 20-year-old Long led from the start to lower her own world mark by 0.43 to 1:05.63, with Great Britain's Heather Frederiksen second.
Long said: 'It's the first time I've really, really cried after a race. The reason was because when I was 12 years old, I wasn't even expected to go to the Paralympics.
'I won my first gold medal at 100m Freestyle aged 12, so this means everything to me. It's my third gold medal in this event and I'm so happy and so proud of myself.'
She added: 'I love what I do, I love getting in the water. Sometimes it's not exactly fun, there are moments in the water when I'm like "what am I doing? This is so nerve-wracking". It's intense in there, it's hard, but once I get in that water, everything melts away. That's my home, that's where I'm a mermaid. That's my place.'
Wang Yinan won the men's equivalent.
Today was also an extraordinary one for home favourite Josef Craig, who lowered his best by more than 11 seconds to twice set a new world record in the 400m Freestyle - S7.
Matthew Cowdrey and Natalie du Toit claimed gold in the men's and women's 200 Individual Medley - SM9, while Yasuhiro Tanaka and Michelle Alonso Morales were successful in the 100m Breaststroke - SB14 events.
There were also wins for Juan Reyes and Lisette Teunissen in the men's and women's 50m Backstroke - S4 events respectively, and Hennadii Boiko in the men's 50m Backstroke - S1.
Daniel Dias stormed to victory in the men's 50m Backstroke - S5, with Andre Brasil and Sophie Pascoe taking the 100m Freestyle - S10 titles.
In the men's 100m Butterfly - S11 race, Viktor Smyrnov won in a time of 1:03.32.