Ennis started the party with a dominant victory in the Heptathlon before Rutherford leapt to victory in the Long Jump, the 25-year-old's winning effort of 8.31m coming at precisely the same time as Ennis was being introduced to the fans before her final event, the 800m.
And Farah then rounded off three astonishing triumphs in the space of 45 minutes with a blistering 53-second last lap in the 10,000m, to take gold ahead of training partner Galen Rupp.
Ennis had a commanding lead going into the 800m but still stormed to victory to improve her national record to 6,955 points and win from Germany's Lilli Schwarzkopf and Tatyana Chernova.
'I can't believe I've had the opportunity to come to my first Games in London and won an Olympic Gold medal. It's unbelievable,' said 26-year-old Ennis, who missed the Beijing 2008 Games after suffering a career-threatening foot injury.
Rutherford, who heads the world rankings in 2012, took the lead in the second round with a jump of 8.21m and was never overtaken, the 25-year-old then jumping 8.31m in the fourth round to extend his lead.
Australia's Mitchell Watt took silver with 8.16m and American Will Claye bronze with 8.12m.
World 5,000m champion Farah had thought his race would not come down to a last-lap burnout as rivals feared his sprinting speed, but in the end that was what happened and Farah hit the front at the bell to time his finish to perfection.
Shelly-Ann Fraser-Pryce defended her Olympic 100m title.
Fraser-Pryce edged out world champion Carmelita Jeter to win in a time of 10.75, with the USA’s Jeter just 0.03 behind and Jamaica's Veronica Campbell-Brown taking bronze in 10.81.
In the morning, defending champion Usain Bolt strolled into the semi-finals of the men’s 100m in 10.09, while South Africa's Oscar Pistorius became the first double amputee to compete in the Olympic and Paralympic Games and the 'Blade Runner' made the semi-finals of the 400m as defending champion LaShawn Merritt crashed out with a hamstring injury.
British success was not confined to the track, with the rowers and cyclists setting the stage for the evening heroics.
At Eton Dorney, Katherine Copeland and Sophie Hosking won the women's Lightweight Double Sculls with the men’s Four also going the way of the hosts.
Zac Purchase and Mark Hunter narrowly failed to make it a hat-trick after losing out to the Danish pair of Mads Rasmussen and Rasmus Quist in the men's Lightweight Double Sculls.
Czech Republic's Miroslava Knapkova won gold in the women's Single Sculls.
In the Velodrome, the British women’s Team Pursuit team defeated the USA in the final.
Britain have won four of the five gold medals on offer after three days of Track Cycling, with five events to come, after Joanna Rowsell, Dani King and Laura Trott clocked 3:14.051 to triumph with a sixth consecutive world record in the three-woman, three-kilometre event.
The Olympic Swimming programme came to an end with Michael Phelps bowing out with the 18th gold medal of his record-breaking career.
The USA superstar, in the last race of his career, swam the butterfly leg as his country claimed gold in the 4 x 100m Medley Relay final.
The USA also won the women’s 4 x 100m Medley Relay while, earlier, China's Sun Yang took the gold and broke the world record in the men’s 1500m Freestyle.
Serena Williams became the first woman to claim the Tennis career Golden Slam in both Singles and Doubles after a ruthless demolition of Maria Sharapova at Wimbledon.
No player apart from German Steffi Graf had previously won all four grand slam events and an Olympic gold in the Singles.
Williams’ 6-0 6-1 win over Russian Sharapova saw her join Graf in achieving that feat, although she can also claim the added distinction of having also done it in the Doubles alongside sister Venus.
'I didn't expect this. Oh my gosh. I have a gold medal in singles,' Williams said.
'I got the gold. I'm just so happy.'
Victoria Azarenka took the bronze after beating Maria Kirilenko 6-3 6-4, while the American Bryan brothers - Mike and Bob - won the men’s Doubles by beating French duo Michael Llodra and Jo-Wilfried Tsonga.
Nicola Spirig won Triathlon gold for Switzerland after edging out Sweden’s Lisa Norden in a photo finish.
Jamie Lynn Gray of the USA was a runaway winner of the women’s 50m Rifle 3 Positions Shooting final, 4.4 ahead of Serbia’s Ivana Maksimovic with Czech Adela Sykorova third.
China's Li Xuerui completed her dream run at Wembley Arena by outbattling her illustrious compatriot Wang Yihan to claim Olympic Badminton gold.
The 21-year-old failed to convert two match points in the second game but managed to summon enough energy to clinch a draining women's Singles final 21-15 21-23 21-17.
Italian Jessica Rossi won gold in the Trap Shooting, Canada’s Rosannagh MacLennan claimed the women’s Individual Trampoline crown and China’s Chen Ding won the men’s 20km Race Walk.
The men’s Football semi-finals will see Japan take on Mexico and South Korea meet Brazil.