A few moments after watching Ed Clancy, Geraint Thomas, Peter Kennaugh and Steven Burke clock a world 3:51.659 to triumph by a supreme margin ahead of arch-rivals Australia in the men's Team Pursuit, Pendleton delivered in her least favoured event.
In the event, which begins behind a motorised Derny bike, the 31-year-old - who is set to retire after London 2012 - stormed to victory.
Guo Shuang of China was second, with Lee Wai Sze of Hong Kong third.
The win sparked jubilant scenes from coach Jan van Eijden, British Cycling Performance Director Dave Brailsford and Head Coach Shane Sutton and was Britain's third gold medal of the Track Cycling programme after Chris Hoy, Philip Hindes and Jason Kenny won the men's Team Sprint yesterday.
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