Ennis broke the British record and smashed her personal best with an exhilarating run of 12.54 in the hurdles, a time which gave American Dawn Harper Olympic gold in the Individual event in Beijing four years ago.
The 26-year-old took 0.25 off her PB and 0.02 off the previous national record set by hurdles specialist Tiffany Porter, while it was also the fastest ever hurdles time recorded in a Heptathlon.
That gave Ennis an 11-point lead over Canada's Jessica Zelinka - whose own time of 12.65 was an impressive PB - which became a total of 2,249 and 25-point lead over the USA's Hyleas Fountain after the high jump.
Ennis cleared 1.86m and was agonisingly close to also getting over 1.89m, a height which was cleared by team-mate Katarina Johnson-Thompson for a new personal best to leave the 19-year-old - who ran 13.48 in the hurdles - third, 103 points behind Ennis.
Reigning Olympic champion Natallia Dobrynska was 193 points behind Ennis in 12th, with world champion Tatyana Chernova 218 points off the pace in 16th.