The fast-emerging Czech duo have begun to make their mark on the World Tour in recent months and have underlined their potential in London this week, though few would have backed them to prevail in this last-16 encounter.
Yet the Europeans looked comfortable as they cantered to the first set and then went close to wrapping the match up in two before the Brazilians took it to a decider.
With Kolocova impressive on defence and Slukova making winning spikes, the shock result eventually arrived as the Czechs ran out 21-16 20-22 15-9 winners in 58 minutes.
Next up for the Czech Republic is a quarter-final clash with Jennifer Kessy and April Ross of the United States.
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